GADD153/CHOP Antibody

Immunocytochemistry/Immunofluorescence: GADD153/CHOP Antibody [NBP2-98741] - Immunofluorescence staining of GADD153/CHOP in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum. Then incubated with rabbit anti-human GADD153/CHOP polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human GADD153/CHOP polyclonal antibody and antigen (Figure B) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.